ncRNA name | hsa-let-7b |
ncRNA Category | miRNA |
Disease name | retinoblastoma (RB) |
Species | Homo sapiens |
Tissues/Cell_line | RB samples; RB cell line Y79 and WERI-RB1; breast cancer cell lines MDA-MB-453 and MCF-7; human retinal Müller glial cell line MIO-M1; human thyroid follicular epithelial cell line Nthy-ori 3-1 |
Methods | qRT-PCR, luciferase reporter assay, MTT assay, Western blot, immunofluorscence |
Expression pattern | down-regulated |
Functional description | let-7b showed consistent down regulation in both the retinoblastoma cell lines when compared to other let-7 family members and showed high up-regulation in Nthy-ori 3-1. Our results showed that the EGP2-promoter HSV-TK (EGP2-TK) construct with 2 or 4 copies of let-7b miRNA targets expressed TK gene only in Y79, WERI-Rb-1, while the TK gene did not express in MIO-M1. |
PubMed ID | 24391761 |
Year | 2013 |
Title | Targeted expression of suicide gene by tissue-specific promoter and microRNA regulation for cancer gene therapy. |
Drug-related ncRNA | NO |
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